Human TPSAB1 / Mast Cell Tryptase

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

AA1

Unconjugated

Protein G purified

Unmodified

Human mast cell tryptase purified from human lung tissue.

Recognizes human mast cell tryptase, both isoforms. Is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology. Tryptases are the products of a number of and form the major neutral protease present in mast cells secreted in response to infection and injury. Mast cell tryptase has an important role in the pathology of inflammatory diseases, especially asthma through bronchoconstriction.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• IHC - Frozen
• Immunofluorescence
• ELISA

Immunohistochemistry: LS-B2347 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2347 was determined to be 2.5 ug/ml. Histology Positive Control Tissue: Tonsil.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q15661 NCBI: NM_003294 NP_003285.2