Target
Mouse TPSAB1 / Mast Cell Tryptase
Synonyms
TPSAB1 | Alpha tryptase | Mast cell alpha II tryptase | Tryptase alpha-1 | Tryptase beta I | Tryptase-III | TPSB1 | Tryptase alpha II | TPS1 | TPS2 | Tryptase beta-1 | Tryptase-1 | Tryptase-I | Mast cell beta I tryptase | Tryptase alpha/beta 1 | Tryptase alpha/beta-1 | Tryptase beta 1 | Tryptase I
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
NS0-derived, recombinant murine Tryptase beta1 (aa29-273).
Specificity
Recognizes murine Tryptase, beta1 in direct ELISAs and Western Blots. Shows ~10% cross-reactivity with recombinant human TPSB2, 5% cross-reactivity with recombinant human TPS1 and less than 1% cross-reactivity with recombinant human TPSG1 and recombinant murine MCPT1.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- Immunoprecipitation
Usage
Suitable for use Western Blot and Immunoprecipitation. Western Blot: 0.1-0.2 ug/ml. The detection limit for recombinant murine Tryptase beta1 is ~1 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: Immunoprecipitates recombinant, murine Tryptase beta1 from conditioned media of transfected NS0 cells. The recovered recombinant murine Tryptase beta1 can be detected by Western Blot analysis using a monoclonal antibody.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute in 500 ul sterile PBS
Storage
Lyophilized and resuspended forms: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TPSAB1 / Mast Cell Tryptase