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Catalog Number	Size	Price
LS-C662095-10	10 µg	$318
LS-C662095-100	100 µg	$470

IL6R / IL6 Receptor Antibody - Western blot analysis of IL6R using anti-IL6R antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6R antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL6R at approximately 52KD. The expected band size for IL6R is at 52KD.

Polyclonal Rabbit anti‑Human IL6R / IL6 Receptor Antibody (aa379‑419, WB) LS‑C662095

Name: Anti-IL6R / IL6 Receptor Antibody | Rabbit anti-Human Polyclonal WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662095-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

IL6R / IL6 Receptor Rabbit anti-Human Polyclonal (aa379-419) Antibody

Application:

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C662095-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

IL6R / IL6 Receptor Rabbit anti-Human Polyclonal (aa379-419) Antibody

Application:

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

IL6 Receptor antibody LS-C662095 is an unconjugated rabbit polyclonal antibody to human IL6 Receptor (IL6R) (aa379-419). Validated for WB.

Target

Human IL6R / IL6 Receptor

Synonyms

See All IL6R / IL6 Receptor Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the C-Terminus of human IL6R (379-419 aa LLCIAIVLRFKKTWKLRALKEGKTSMHPPYSLGQLVPERPR).

Epitope

aa379-419

Specificity

Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.

Applications

Western blot

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About IL6R / IL6 Receptor

Uniprot: P08887 NCBI: NM_000565 NP_000556.1