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Catalog Number Size Price
LS-C352492-100 100 µl (1 mg/ml) $315 
SMAD3 Antibody - Immunofluorescent analysis of SMAD3 (pS204) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
SMAD3 Antibody - Western blot analysis of SMAD3 (pS204) expression in HeLa UV-treated (A); SP2/0 UV-treated (B); H9C2 UV-treated (C) whole cell lysates.
SMAD3 Antibody - Immunofluorescent analysis of SMAD3 (pS204) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
SMAD3 Antibody - Western blot analysis of SMAD3 (pS204) expression in HeLa UV-treated (A); SP2/0 UV-treated (B); H9C2 UV-treated (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human SMAD3 Antibody (phospho‑Ser204, IF, WB) LS‑C352492

Polyclonal Rabbit anti‑Human SMAD3 Antibody (phospho‑Ser204, IF, WB) LS‑C352492

Antibody:
SMAD3 Rabbit anti-Human Polyclonal (pSer204) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse, Rat, Pig, Sheep, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$315
LS-C352492-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SMAD3 Rabbit anti-Human Polyclonal (pSer204) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse, Rat, Pig, Sheep, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD3 antibody LS-C352492 is an unconjugated rabbit polyclonal antibody to SMAD3 (pSer204) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IP and WB.
Target
Human SMAD3
Synonyms
SMAD3 | HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | HSMAD3 | Mad protein homolog | SMAD 3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Pig, Sheep, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity chromatography
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human SMAD3.
Epitope
pSer204
Specificity
Recognizes endogenous levels of SMAD3 (pS204) protein.
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD3
P84022 NM_005902 NP_005893.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/22/2024