Mouse THBD / CD141 / Thrombomodulin

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

461714

Unconjugated

Protein G purified

Unmodified

NS0-derived rmThrombomodulin. (aa 17 - 517).

aa17-517

Recognizes mouse Thrombomodulin.

• IHC
• IHC - Frozen
• ICC (10 µg/ml)
• Western blot (1 - 2 µg/ml)
• Flow Cytometry (25 µg/ml)
• ELISA

Suitable for use in Flow Cytometry, ELISA, Western Blot Immunohistochemistry and Immunocytochemistry. Flow Cytometry: 25 ug/ml; Use 10 ul to label 1-2.5x10^5 bEnd.3 cells in a total reaction volume < 200ul. ELISA (Direct): 0.5-1.0 ug/ml The detection limit is ~5 ng/well. Western Blot: 1-2 ug/ml Using a colorimetric detection system, the detection limit is ~25 ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5-50 fold. Immunohistochemistry: 25 ug/ml. Used with frozen sections of normal mouse kidney and heart fixed with 85 mM Na2HPO4, 75 mM KH2P04, 4% paraformaldehyde, and 14% (v/v) saturated picric acid, pH 6.9. Immunocytochemistry: 10 ug/ml. Used with bEnd.3 cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. Between each step, cells were washed with PBS, BSA.

Lyophilized from PBS, 5% Trehalose

Reconstitute in 200 ul sterile PBS

Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P07204 NCBI: NM_000361 NP_000352.1