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| Catalog Number | Size | Price |
|---|---|---|
| LS-B4674-100 | 100 µl (0.5 mg/ml) | $485 |
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PathPlus™ Polyclonal Rabbit anti‑Human CDH1 / E Cadherin Antibody (aa503‑552, IHC, WB) LS‑B4674
PathPlus™ Polyclonal Rabbit anti‑Human CDH1 / E Cadherin Antibody (aa503‑552, IHC, WB) LS‑B4674
Note: This antibody replaces LS-C111310
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
E-cadherin (CDH1) is a calcium-depending glycoprotein that is involved in cell to cell adhesion and is expressed on the surface of epithelial tissues. Loss of e-cadherin is associated with decreased cell adhesion, increased cellular motility, invasion and poor prognosis in carcinomas, particularly lobular carcinomas of the breast. In immunohistochemistry, e-cadherin is positive in normal breast ducts, the epithelium of the GI tract, hepatocytes and bile ducts. In cancer, it is positive in breast ductal carcinoma (but not lobular), esophageal adenocarcinoma and squamous carcinoma, and bladder transitional cell carcinoma.
References: Eur J Cancer 2000, 36:1607; Cell. 2015 Oct 8;163(2):506-19, PMID: 26451490; Breast Cancer Res. 2017 Jul 27;19(1):86, PMID: 28750639; Oncotarget 2017 Jan; 8.1; 16445-16455, PMID: 28147315; Oncotarget. 2016 Nov; 7.49; 80655-80663, PMID: 27811364;
Target
Human CDH1 / E Cadherin
Synonyms
CDH1 | CD324 | CDHE | Cell-CAM 120/80 | E Cadherin | E-Cadherin | ECAD | Epithelial cadherin | Arc-1 | UVO | Cadherin-1 | CAM 120/80 | CD324 antigen | LCAM | Uvomorulin
Host
Rabbit
Reactivity
Human, Rat
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide located between aa503-552 of human CDH1 (P12830, NP_004351). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant (100%); Bovine, Bat, Rabbit, Horse, Pig (92%); Zebrafish (84%); Mouse, Rat (78%).
Epitope
aa503-552
Specificity
Human CDH1 / E Cadherin
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.2 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CDH1 / E Cadherin
Validation
Anti-E Cadherin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-E Cadherin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-E Cadherin antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-E Cadherin antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
PathPlus™ CDH1 / E Cadherin Antibody (aa503-552) for IHC, WB/Western LS-B4674 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC - Paraffin, ELISA
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human, Monkey, Rat, Bovine, Dog, Pig, Rabbit, Zebrafish
Applications:
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/27/2024
Date Requested: 12/27/2024
