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Catalog Number	Size	Price
LS-B16416-50	50 µl (1 mg/ml)	$460

CDH1 / E Cadherin Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

CDH1 / E Cadherin Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

CDH1 / E Cadherin Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

CDH1 / E Cadherin Antibody - Immunofluorescent analysis of E Cadherin staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

CDH1 / E Cadherin Antibody - Western blot analysis of E Cadherin expression in HEK293T (A); PC12 (B); A431 (C); MCF7 (D); C2C12 (E) whole cell lysates.

CDH1 / E Cadherin Antibody - Immunoprecipitation of E Cadherin from 0.5mg HEK293F whole cell extract lysate using 5ug of Anti-E Cadherin Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min HEK293F whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-E Cadherin Antibody.

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PathPlus™ Polyclonal Rabbit anti‑Human CDH1 / E Cadherin Antibody (C‑Terminus, IHC, IF, WB) LS‑B16416

Name: PathPlus™ CDH1 / E Cadherin Polyclonal Antibody Rabbit | LSBio
Brand: Lifespan BioSciences
SKU: LS-B16416-50
Price: 460.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

Cancer Pathology Cancer

(5.0) Rating

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Note: This antibody replaces LS-C351977

Antibody:

CDH1 / E Cadherin Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC-P, ICC, IF, WB, IP, Flo

Reactivity:

Human, Monkey, Rat, Bovine, Dog, Pig, Rabbit, Zebrafish

Format:

Unconjugated, Unmodified

$460

LS-B16416-50

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(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CDH1 / E Cadherin Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC-P, ICC, IF, WB, IP, Flo

Reactivity:

Human, Monkey, Rat, Bovine, Dog, Pig, Rabbit, Zebrafish

Format:

Unconjugated, Unmodified

Specifications

Description

E-cadherin (CDH1) is a calcium-depending glycoprotein that is involved in cell to cell adhesion and is expressed on the surface of epithelial tissues. Loss of e-cadherin is associated with decreased cell adhesion, increased cellular motility, invasion and poor prognosis in carcinomas, particularly lobular carcinomas of the breast. In immunohistochemistry, e-cadherin is positive in normal breast ducts, the epithelium of the GI tract, hepatocytes and bile ducts. In cancer, it is positive in breast ductal carcinoma (but not lobular), esophageal adenocarcinoma and squamous carcinoma, and bladder transitional cell carcinoma. References: Eur J Cancer 2000, 36:1607; Cell. 2015 Oct 8;163(2):506-19, PMID: 26451490; Breast Cancer Res. 2017 Jul 27;19(1):86, PMID: 28750639; Oncotarget 2017 Jan; 8.1; 16445-16455, PMID: 28147315; Oncotarget. 2016 Nov; 7.49; 80655-80663, PMID: 27811364;

Target

Human CDH1 / E Cadherin

Synonyms

See All CDH1 / E Cadherin Antibodies

Host

Rabbit

Reactivity

Human, Monkey, Rat, Bovine, Dog, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human E Cadherin.

Epitope

C-Terminus

Specificity

Recognizes endogenous levels of E Cadherin protein.

Applications

IHC - Paraffin (6 µg/ml)
ICC (1:100 - 1:500)
Immunofluorescence (1:100 - 1:500)
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:10 - 1:100)
Flow Cytometry (1:100 - 1:200)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CDH1 / E Cadherin

Uniprot: P12830 NCBI: NM_004360 NP_004351.1