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| Catalog Number | Size | Price |
|---|---|---|
| LS-B11248-100 | 100 µg (1 mg/ml) | $460 |
PathPlus™ Polyclonal Rabbit anti‑Human CX3CL1 / Fractalkine Antibody (IHC, WB) LS‑B11248
PathPlus™ Polyclonal Rabbit anti‑Human CX3CL1 / Fractalkine Antibody (IHC, WB) LS‑B11248
Note: This antibody replaces LS-C104505, LS-C42779
Antibody:
CX3CL1 / Fractalkine Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CX3CL1 / Fractalkine Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
Fractalkine (CX3CL1) is a chemokine with a unique CX3C motif. Fractalkine is synthesized in endothelial cells as a membrane protein, and the N-terminal domain containing a CX3C motif is cleaved and secreted. Fractalkine is the first described cell-surface anchored chemokine and has potent mononuclear cell-directed adhesion and chemotactic properties. Fractalkine is involved in the pathogenesis of various chronic inflammatory diseases, such as rheumatoid arthritis and atherosclerosis.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Human CX3CL1 / Fractalkine
Synonyms
CX3CL1 | ABCD-3 | C-X3-C motif chemokine 1 | C3Xkine | Chemokine ntt | CXC3C | FKN | Fractalkine | Neurotactin | CXC3 | NTN | SCYD1 | Small-inducible cytokine D1
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated.
Also available Unconjugated or conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human Fractalkine.
Specificity
Human Fractalkine
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.5 - 2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
ELISA: To detect hFractalkine by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Fractalkine (LS-C104345) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFractalkine. Western Blot: To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CX3CL1 / Fractalkine
Validation
Anti-CX3CL1 / Fractalkine antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CX3CL1 / Fractalkine antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
PathPlus™ CX3CL1 / Fractalkine Antibody for IHC, WB/Western, ELISA LS-B11248 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC - Paraffin, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
Date Requested: 12/22/2024
