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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5625-100 | 100 µl (0.5 mg/ml) | $485 |
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PathPlus™ Polyclonal Rabbit anti‑Human TJP1 / ZO‑1 Antibody (aa935‑984, IHC, WB) LS‑B5625
PathPlus™ Polyclonal Rabbit anti‑Human TJP1 / ZO‑1 Antibody (aa935‑984, IHC, WB) LS‑B5625
Note: This antibody replaces LS-C110185
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
TJP1 (Tight junction protein-1, Zonula occludens-1, ZO-1) is a scaffold protein involved in tight junction assembly, anchoring and stabilization. It functions in cell-cell junction signal transduction and in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. TJP1 is upregulated in and drug-resistant multiple myeloma, and is associated with a higher risk subset of cancers. TJP1 has been found to be upregulated in drug-resistant tumors. In immunohistochemistry of normal tissue, TJP1 has membranous positivity in endothelial cells, glandular epithelia, renal glomeruli, sertoli cells, and is expressed in most tissues throughout the body.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804; Oncoscience. 2017 Jul; 4(7-8): 79–94, PMID: 28966941
Target
Human TJP1 / ZO-1
Synonyms
TJP1 | ZO-1 | Zona occludens protein 1 | Zona occludens 1 | Zonula occludens protein 1 | Tight junction protein 1 | ZO1 | Tight junction protein ZO-1 | Zonula occludens 1 protein
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide located between aa935-984 of human TJP1 (Q07157, NP_783297). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Rabbit (100%); Rat, Dog, Bat, Horse, Pig (92%); Mouse (86%); Guinea pig (85%).
Epitope
aa935-984
Specificity
Human TJP1 / ZO-1
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.2 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TJP1 / ZO-1
Validation
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testins: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testins: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-ZO-1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ZO-1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
PathPlus™ TJP1 / ZO-1 Antibody (aa935-984) for IHC, WB/Western LS-B5625 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Urothelial cell expansion and differentiation are improved by exposure to hypoxia. Stéphane Chabaud, Ingrid Saba, Clément Baratange, Brice Boiroux, Maude Leclerc, Alexandre Rousseau, Sara Bouhout, Stéphane Bolduc. Journal of tissue engineering and regenerative medicine. 2017 November;11:3090-3099.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Species:
Human, Monkey, Mouse, Rat, Dog
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Species:
Human, Monkey, Mouse, Rat, Dog
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat, Dog, Guinea pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
