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| Catalog Number | Size | Price |
|---|---|---|
| LS-G330-5 | 5 µg | $268 |
| LS-G330-25 | 25 µg | $349 |
Human TNFSF12 / TWEAK Protein (Recombinant) - LS-G330
Human TNFSF12 / TWEAK Protein (Recombinant) - LS-G330
Description:
TNFSF12 / TWEAK Protein LS-G330 is a Recombinant Human TNFSF12 / TWEAK produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.1 ng/µg of protein (less than 1EU/µg). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
TNFSF12 / TWEAK Protein LS-G330 is a Recombinant Human TNFSF12 / TWEAK produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.1 ng/µg of protein (less than 1EU/µg). For Research Use Only
Specifications
Type
Recombinant Protein
Target
TNFSF12 / TWEAK
Synonyms
TNFSF12 | APO3/DR3 ligand | APO3L | DR3LG | APO3 ligand | TWEAK
Species
Human
Modifications
Unmodified.
Also available Animal-free.
Conjugations
Unconjugated
Predicted Molecular Weight
17.0 kDa
AA Sequence
MKGRKTRARRAIAAHYEVHPRPGQDGAQAGVDGTVSGWEEARINSSSPLRYNRQIGEFIVTRAGLYYLYCQVHFDEGKAVYLKLDLLVDGVLALRCLEEFSATAASSLGPQLRLCQVSGLLALRPGSSLRIRTLPWAHLKAAPFLTYFGLFQVH
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
Assay 1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10 ng/ml. Assay 2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-gamma. The ED50 for this effect is between 30-45 ng/ml.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Sterile filtered, lyophilized from 10 mM sodium phosphate, pH 7.5, 0.1 M L-Arg
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in 10 mM Sodium Phosphate pH 7.5 to a concentration of 1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNFSF12 / TWEAK
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Popular TNFSF12 / TWEAK Proteins
Source:
E. coli
Tag:
6His, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
