Target
Human CASP3 / Caspase 3
Synonyms
CASP3 | Apopain | CASP-3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama
Reactivity
Human, Hamster
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-RDVSKEDHSKRS, from the internal region of the protein sequence according to NP_004337.2NP_116786.1.
Specificity
Human Caspase 3. This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).
Applications
- Immunofluorescence (10 µg/ml)
- Western blot (0.5 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 30kD band observed in lysates of cell line MOLT4 (calculated MW of 31.6kD according to NP_004337.2;). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 65kD. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee