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Catalog Number Size Price
LS-C747198-20 20 µl $287 
LS-C747198-100 100 µl (1.33 mg/ml) $431 
CASP3 / Caspase 3 Antibody - Immunohistochemistry of paraffin-embedded human gastric cancer using CASP3 antibody at dilution of 1:100 (40x lens).
CASP3 / Caspase 3 Antibody - Immunohistochemistry of paraffin-embedded rat brain using CASP3 antibody at dilution of 1:100 (40x lens).
CASP3 / Caspase 3 Antibody - Immunohistochemistry of paraffin-embedded rat lung using CASP3 antibody at dilution of 1:100 (40x lens).
CASP3 / Caspase 3 Antibody - Immunofluorescence analysis of Jurkat cells using CASP3 antibody at dilution of 1:100. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours (left). Blue: DAPI for nuclear staining.
CASP3 / Caspase 3 Antibody - Western blot analysis of extracts of Jurkat cells, using CASP3 antibody. Jurkat cells treated by Stauroporine. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
CASP3 / Caspase 3 Antibody - Immunohistochemistry of paraffin-embedded human gastric cancer using CASP3 antibody at dilution of 1:100 (40x lens).
CASP3 / Caspase 3 Antibody - Immunohistochemistry of paraffin-embedded rat brain using CASP3 antibody at dilution of 1:100 (40x lens).
CASP3 / Caspase 3 Antibody - Immunohistochemistry of paraffin-embedded rat lung using CASP3 antibody at dilution of 1:100 (40x lens).
CASP3 / Caspase 3 Antibody - Immunofluorescence analysis of Jurkat cells using CASP3 antibody at dilution of 1:100. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours (left). Blue: DAPI for nuclear staining.
CASP3 / Caspase 3 Antibody - Western blot analysis of extracts of Jurkat cells, using CASP3 antibody. Jurkat cells treated by Stauroporine. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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Polyclonal Rabbit anti‑Human CASP3 / Caspase 3 Antibody (IHC, IF, WB) LS‑C747198

Polyclonal Rabbit anti‑Human CASP3 / Caspase 3 Antibody (IHC, IF, WB) LS‑C747198

Antibody:
CASP3 / Caspase 3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C747198-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CASP3 / Caspase 3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 3 antibody LS-C747198 is an unconjugated rabbit polyclonal antibody to Caspase 3 (CASP3) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human CASP3 / Caspase 3
Synonyms
CASP3 | Apopain | CASP-3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human CASP3 (NP_004337.2). FHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFII
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 31kDa, while the observed MW by Western blot was 17kDa, 35kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP3 / Caspase 3
P42574 NM_004346 NP_004337.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/21/2024