Target
Human CASP3 / Caspase 3
Synonyms
CASP3 | Apopain | CASP-3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Alexa Fluor 488
Purification
Protein A purified
Immunogen
Synthetic peptide corresponding to N-Terminus residues adjacent to Asp175 of human caspase-3 (KLH).
Specificity
Detects endogenous levels of the large fragment (17/19 kD) of activated caspase-3 resulting from cleavage adjacent to aspartic acid 175. Does not recognize full length caspase-3 or other cleaved caspases. Species cross-reactivity: Mouse and rat.
Applications
- ICC (1:10)
- Immunofluorescence
- Flow Cytometry (1:10)
- ELISA
Usage
Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, and Immunofluorescence. Flow Cytometry: 1:10. Immunocytochemistry: 1:10. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 2 mg/ml BSA, 0.1% Sodium Azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee