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| Catalog Number | Size | Price |
|---|---|---|
| LS-C12477-100 | 100 µl | $709 |
Monoclonal Rabbit anti‑Human CASP3 / Caspase 3 Antibody (N‑Terminus, IHC, IF, WB) LS‑C12477
Monoclonal Rabbit anti‑Human CASP3 / Caspase 3 Antibody (N‑Terminus, IHC, IF, WB) LS‑C12477
Antibody:
CASP3 / Caspase 3 Rabbit anti-Human Monoclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CASP3 / Caspase 3 Rabbit anti-Human Monoclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Caspase 3 antibody LS-C12477 is an unconjugated rabbit monoclonal antibody to human Caspase 3 (CASP3) (N-Terminus). Validated for Flow, IF, IHC, IP and WB.
Target
Human CASP3 / Caspase 3
Synonyms
CASP3 | Apopain | CASP-3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Monoclonal
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Immunogen
Synthetic peptide (KLH coupled) corresponding to N-Terminus residues adjacent to (Asp175) in human caspase-3.
Epitope
N-Terminus
Specificity
Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- Immunofluorescence
- Western blot (1:1000)
- Immunoprecipitation (1:50)
- Flow Cytometry (1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence. Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunohistochemistry (Frozen): 1:200 using 3% formaldehyde as fixative. Immunohistochemistry (Paraffin): 1:200. Antigen retrieval: heat in citrate buffer, pH 8.0. Immunofluorescence (IC): 1:200. Flow Cytometry: 1:200.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CASP3 / Caspase 3
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Rabbit, Chicken, Yeast
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Frozen, ICC, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Gerbil
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Pig, Rabbit, Sheep
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, Western blot, Flow Cytometry, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
