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Antibodies > IL7 > LS-C313460
 
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Catalog Number Size Price
LS-C313460-10 10 µg $318 
LS-C313460-100 100 µg (0.5 mg/ml) $470 
IL7 Antibody - Western blot analysis of IL-7 using anti-IL-7 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-7 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL-7 at approximately 20KD. The expected band size for IL-7 is at 20KD.
IL7 Antibody - Anti-human IL7 antibody, Western blotting Lane 1: Recombinant Human IL-7 Protein 10ng Lane 2: Recombinant Human IL-7 Protein 5ng Lane 3: Recombinant Human IL-7 Protein 2
IL7 Antibody - WB of IL7 antibody. Lane 1: Recombinant Human IL-7 Protein 10ng. Lane 2: Recombinant Human IL-7 Protein 5ng. Lane 3: Recombinant Human IL-7 Protein 2.5ng. Lane 4: Recombinant Human IL-7 Protein 1.25ng..
IL7 Antibody - Western blot analysis of IL-7 using anti-IL-7 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-7 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL-7 at approximately 20KD. The expected band size for IL-7 is at 20KD.
IL7 Antibody - Anti-human IL7 antibody, Western blotting Lane 1: Recombinant Human IL-7 Protein 10ng Lane 2: Recombinant Human IL-7 Protein 5ng Lane 3: Recombinant Human IL-7 Protein 2
IL7 Antibody - WB of IL7 antibody. Lane 1: Recombinant Human IL-7 Protein 10ng. Lane 2: Recombinant Human IL-7 Protein 5ng. Lane 3: Recombinant Human IL-7 Protein 2.5ng. Lane 4: Recombinant Human IL-7 Protein 1.25ng..
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Polyclonal Rabbit anti‑Human IL7 Antibody (aa26‑177, WB) LS‑C313460

Polyclonal Rabbit anti‑Human IL7 Antibody (aa26‑177, WB) LS‑C313460

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Note: This antibody replaces LS-C388692
Antibody:
IL7 Rabbit anti-Human Polyclonal (aa26-177) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313460-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IL7 Rabbit anti-Human Polyclonal (aa26-177) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL7 antibody LS-C313460 is an unconjugated rabbit polyclonal antibody to human IL7 (aa26-177). Validated for ELISA and WB.
Target
Human IL7
Synonyms
IL7 | IL-7 | Interleukin-7 | Interleukin 7
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human IL-7 recombinant protein(Position: D26-H177).
Epitope
aa26-177
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL7
P13232 NM_000880 NP_000871.1

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Featured Products

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IL7 Antibody LS‑C8012
Species: Mouse
Applications: Western blot, ELISA, Functional Assay, Neutralization
IL7 Antibody - Western Blot (reducing) of Interleukin 7 / IL-7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL7 Antibody LS‑C104554
Species: Human
Applications: Western blot, ELISA
IL7 Antibody - anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
IL7 Antibody (HRP) LS‑C153806
Species: Human
Applications: IHC, Western blot, ELISA
IL7 Antibody - Biotin Rabbit Anti-Human IL-7 Antibody - Western Blot. Western Blot showing detection of Human IL-7. 50 ng of Human IL-7 (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20?, Anti-Human IL-7 (RABBIT) Antibody Biotin Conjugated Anti-Human IL-7 (RABBIT) Antibody Biotin Conjugated - 209-406-B94 secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20? and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 17 kD molecular weight position expected for Human IL-7. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL7 Antibody (Biotin) LS‑C154121
Species: Human
Applications: IHC, Western blot, ELISA
IL7 Antibody - Western Blot (reducing) of Interleukin 7 / IL-7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL7 Antibody LS‑C104812
Species: Mouse
Applications: Western blot, ELISA
IL7 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ IL7 Antibody LS‑B14351
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
See all IL7 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

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