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Catalog Number Size Price
LS-C47546-100 100 µg (0.5 mg/ml) $503 
NALP3 / NLRP3 Antibody - NALP3 / NLRP3 antibody (5µg/ml) staining of paraffin embedded Human Peripheral Blood Leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
NALP3 / NLRP3 Antibody -  Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
NALP3 / NLRP3 Antibody - NALP3 / NLRP3 antibody immunofluorescence analysis of paraformaldehyde fixed U937 cells permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
NALP3 / NLRP3 Antibody - NALP3 / NLRP3 antibody flow cytometric analysis of paraformaldehyde fixed human peripheral blood monocytes (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
NALP3 / NLRP3 Antibody - NALP3 / NLRP3 antibody (5µg/ml) staining of paraffin embedded Human Peripheral Blood Leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
NALP3 / NLRP3 Antibody -  Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
NALP3 / NLRP3 Antibody - NALP3 / NLRP3 antibody immunofluorescence analysis of paraformaldehyde fixed U937 cells permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
NALP3 / NLRP3 Antibody - NALP3 / NLRP3 antibody flow cytometric analysis of paraformaldehyde fixed human peripheral blood monocytes (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Polyclonal Goat anti‑Human NALP3 / NLRP3 Antibody (C‑Terminus, IHC, IF) LS‑C47546

Polyclonal Goat anti‑Human NALP3 / NLRP3 Antibody (C‑Terminus, IHC, IF) LS‑C47546

Antibody:
NALP3 / NLRP3 Goat anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, IF, Flo, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C47546-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NALP3 / NLRP3 Goat anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, IF, Flo, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NLRP3 antibody LS-C47546 is an unconjugated goat polyclonal antibody to human NLRP3 (NALP3) (C-Terminus). Validated for Flow, IF, IHC and Peptide-ELISA.
Target
Human NALP3 / NLRP3
Synonyms
NLRP3 | AGTAVPRL | AII | C1orf7 | Caterpiller protein 1.1 | Cryopyrin | FCAS | FCU | PYPAF1 | NALP3 | CIAS1 | CLR1.1 | MWS
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-EKPELTVVFEPSW, from the C-Terminus of the protein sequence according to NP_001073289.1; NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1.
Epitope
C-Terminus
Specificity
Variants (NP_001073289.1; NP_004886.3) encode the same isoform.
Applications
  • IHC (2.5 µg/ml)
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence (10 µg/ml): Strong expression of the protein seen in the plasma membrane and cytoplasm of U937 cells.
  • Flow Cytometry (10 µg/ml): Human peripheral blood monocyte cells.
  • Peptide Enzyme-Linked Immunosorbent Assay (1:2000): Detection Limit
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • Western blot
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NALP3 / NLRP3
Q96P20 NM_004895 NP_004886.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/25/2024