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Catalog Number	Size	Price
LS-C47546-100	100 µg (0.5 mg/ml)	$503

NALP3 / NLRP3 Antibody - NALP3 / NLRP3 antibody (5µg/ml) staining of paraffin embedded Human Peripheral Blood Leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

NALP3 / NLRP3 Antibody - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

NALP3 / NLRP3 Antibody - NALP3 / NLRP3 antibody immunofluorescence analysis of paraformaldehyde fixed U937 cells permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

NALP3 / NLRP3 Antibody - NALP3 / NLRP3 antibody flow cytometric analysis of paraformaldehyde fixed human peripheral blood monocytes (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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Polyclonal Goat anti‑Human NALP3 / NLRP3 Antibody (C‑Terminus, IHC, IF) LS‑C47546

Name: Anti-NALP3 / NLRP3 Antibody | Goat anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C47546-100
Price: 503.0000 USD
Availability: InStock

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Antibody:

NALP3 / NLRP3 Goat anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, IF, Flo, Peptide-ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$503

LS-C47546-100

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Overview

Antibody:

NALP3 / NLRP3 Goat anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, IF, Flo, Peptide-ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

NLRP3 antibody LS-C47546 is an unconjugated goat polyclonal antibody to human NLRP3 (NALP3) (C-Terminus). Validated for Flow, IF, IHC and Peptide-ELISA.

Target

Human NALP3 / NLRP3

Synonyms

See All NALP3 / NLRP3 Antibodies

Host

Goat

Reactivity

Human (tested or 100% immunogen sequence identity)

Predicted

Rat, Bovine (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-EKPELTVVFEPSW, from the C-Terminus of the protein sequence according to NP_001073289.1; NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1.

Epitope

C-Terminus

Specificity

Variants (NP_001073289.1; NP_004886.3) encode the same isoform.

Applications

IHC (2.5 µg/ml)
IHC - Paraffin (2.5 µg/ml)
Immunofluorescence (10 µg/ml): Strong expression of the protein seen in the plasma membrane and cytoplasm of U937 cells.
Flow Cytometry (10 µg/ml): Human peripheral blood monocyte cells.
Peptide Enzyme-Linked Immunosorbent Assay (1:2000): Detection Limit

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Failed Applications