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Antibodies > NF-L / NEFL > LS-C204558
 
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Catalog Number Size Price
LS-C204558-100 100 µl $376 
NF-L / NEFL Antibody - Mixed neuron/glia cultures from newborn rat brain stained with antibody to peripherin (green) and rabbit polyclonal antibody to NF-L NF-L / NEFL antibody (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the peripherin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.
NF-L / NEFL Antibody - Western blot of whole rat cerebellum homogenate stained with NF-L / NEFL antibody in the left strip, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kDa corresponds to rodent NF-L. Faint bands below this are in vivo degradation products generated by calpain digestion. The major NF-H band in human, cow and bovine run a little slower on SDS-PAGE, at about 70kDa. Right lane shows a strip incubated with a new antibody against neuron specific enolase, which reveals a major band at 47kDa.
NF-L / NEFL Antibody - Mixed neuron/glia cultures from newborn rat brain stained with antibody to peripherin (green) and rabbit polyclonal antibody to NF-L NF-L / NEFL antibody (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the peripherin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.
NF-L / NEFL Antibody - Western blot of whole rat cerebellum homogenate stained with NF-L / NEFL antibody in the left strip, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kDa corresponds to rodent NF-L. Faint bands below this are in vivo degradation products generated by calpain digestion. The major NF-H band in human, cow and bovine run a little slower on SDS-PAGE, at about 70kDa. Right lane shows a strip incubated with a new antibody against neuron specific enolase, which reveals a major band at 47kDa.
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Polyclonal Rabbit anti‑Human NF‑L / NEFL Antibody (IHC, IF, WB) LS‑C204558

Polyclonal Rabbit anti‑Human NF‑L / NEFL Antibody (IHC, IF, WB) LS‑C204558

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Antibody:
NF-L / NEFL Rabbit anti-Human Polyclonal Antibody
Application:
IHC, ICC, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$376
LS-C204558-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NF-L / NEFL Rabbit anti-Human Polyclonal Antibody
Application:
IHC, ICC, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NEFL antibody LS-C204558 is an unconjugated rabbit polyclonal antibody to NEFL (NF-L) from human. It is reactive with human and rat. Validated for ICC, IF, IHC and WB.
Target
Human NF-L / NEFL
Synonyms
NEFL | 68 kDa neurofilament protein | CMT1F | CMT2E | NF68 | NF-L | NFL | Neurofilament subunit NF-L | PPP1R110 | Neurofilament light polypeptide | CMTDIG
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
Pig intermediate filaments were prepared from spinal cords by the method of Delacourte et al., and this cytoskeletal material was dissolved in 6M urea. NF-L was purified by ion exchange chromatography, followed by preparative gel electrophoresis.
Specificity
Human NF-L / NEFL
Applications
  • IHC
  • ICC (1:5000)
  • Immunofluorescence (1:500 - 1:1000)
  • Western blot (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Try at dilutions of 1:500-1:1000 for immunofluorescence, and 1:5000 for ABC or other enzyme linked immunocytochemical procedures. For western blots try at 1:20000.
Presentation
Antiserum
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NF-L / NEFL
P07196 NM_006158 NP_006149.2

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Featured Products

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Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
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NF‑L / NEFL Antibody (clone OTI3G2) LS‑C175341
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Product
NF‑L / NEFL Antibody (aa128‑271, Biotin) LS‑C678794
Species: Human
Applications: ELISA
NF-L / NEFL Antibody - Anti-NF-L / NEFL antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC‑plus™ NF‑L / NEFL Antibody LS‑B10376
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
NF-L / NEFL Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ NF‑L / NEFL Antibody (aa295‑344) LS‑B12562
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
NF-L / NEFL Antibody - Embryonic rat cortical cells grown in tissue culture and stained with NF-L / NEFL antibody (green) and the phosphorylated axonal form of neurofilament NF-H, using NF-H antibody (red). The perikaryal and dendritic neurofilaments in the Large cell in middle are stained with NF-L / NEFL antibody but not with the phosphorylated NF-H antibody. In contrast both antibodies stain axonal neurofilaments which therefore appear orange.
NF‑L / NEFL Antibody (clone 7D1) LS‑C204557
Species: Human, Mouse, Rat, Bovine, Pig
Applications: ICC, Immunofluorescence, Western blot
See all NF-L / NEFL Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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