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Vector Laboratories, Inc.
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Catalog Number Size Price
LS-C344084-10 10 µg $318 
LS-C344084-100 100 µg (0.5 mg/ml) $470 
TJP1 / ZO-1 Antibody - TJP1 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
TJP1 / ZO-1 Antibody - Western blot analysis of TJP1 using anti-TJP1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human K562 whole cell lysates. Lane 5: human PC-3 whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: rat ovary tissue lysates, Lane 8: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TJP1 at approximately 220-240KD. The expected band size for TJP1 is at 195KD.
TJP1 / ZO-1 Antibody - TJP1 antibody Western blot. All lanes: Anti TJP1 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Mouse Liver Tissue Lysate at 50 ug. Lane 3: NRK Whole Cell Lysate at 40 ug. Lane 4: PC-12 Whole Cell Lysate at 40 ug. Lane 5: HELA Whole Cell Lysate at 40 ug. Lane 6: SMMC Whole Cell Lysate at 40 ug. Lane 7: HEPA Whole Cell Lysate at 40 ug. Lane 8: COLO320 Whole Cell Lysate at 40 ug. Predicted band size: 195 kD. Observed band size: 195 kD.
TJP1 / ZO-1 Antibody - TJP1 antibody Western blot. All lanes: Anti TJP1 at 0.5 ug/ml. WB: Recombinant Human TJP1 Protein 0.5ng. Predicted band size: 48 kD. Observed band size: 48 kD.
TJP1 / ZO-1 Antibody - TJP1 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
TJP1 / ZO-1 Antibody - Western blot analysis of TJP1 using anti-TJP1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human K562 whole cell lysates. Lane 5: human PC-3 whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: rat ovary tissue lysates, Lane 8: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TJP1 at approximately 220-240KD. The expected band size for TJP1 is at 195KD.
TJP1 / ZO-1 Antibody - TJP1 antibody Western blot. All lanes: Anti TJP1 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Mouse Liver Tissue Lysate at 50 ug. Lane 3: NRK Whole Cell Lysate at 40 ug. Lane 4: PC-12 Whole Cell Lysate at 40 ug. Lane 5: HELA Whole Cell Lysate at 40 ug. Lane 6: SMMC Whole Cell Lysate at 40 ug. Lane 7: HEPA Whole Cell Lysate at 40 ug. Lane 8: COLO320 Whole Cell Lysate at 40 ug. Predicted band size: 195 kD. Observed band size: 195 kD.
TJP1 / ZO-1 Antibody - TJP1 antibody Western blot. All lanes: Anti TJP1 at 0.5 ug/ml. WB: Recombinant Human TJP1 Protein 0.5ng. Predicted band size: 48 kD. Observed band size: 48 kD.
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Polyclonal Rabbit anti‑Human TJP1 / ZO‑1 Antibody (aa1178‑1527, IHC, WB) LS‑C344084

Polyclonal Rabbit anti‑Human TJP1 / ZO‑1 Antibody (aa1178‑1527, IHC, WB) LS‑C344084

Note: This antibody replaces LS-C389310
Antibody:
TJP1 / ZO-1 Rabbit anti-Human Polyclonal (aa1178-1527) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C344084-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TJP1 / ZO-1 Rabbit anti-Human Polyclonal (aa1178-1527) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ZO-1 antibody LS-C344084 is an unconjugated rabbit polyclonal antibody to ZO-1 (TJP1) (aa1178-1527) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human TJP1 / ZO-1
Synonyms
TJP1 | ZO-1 | Zona occludens protein 1 | Zona occludens 1 | Zonula occludens protein 1 | Tight junction protein 1 | ZO1 | Tight junction protein ZO-1 | Zonula occludens 1 protein
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human TJP1 recombinant protein (Position: H1178-F1527). Human TJP1 shares 82% amino acid (aa) sequence identity with mouse TJP1.
Epitope
aa1178-1527
Specificity
The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TJP1 / ZO-1
Q07157 NM_003257 NP_003248.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024