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Catalog Number Size Price
LS-C331048-20 20 µl $275 
LS-C331048-100 100 µl $389 
TJP1 / ZO-1 Antibody - Western blot analysis of extracts of various cell lines, using TJP1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 15s.

Polyclonal Rabbit anti‑Human TJP1 / ZO‑1 Antibody (IHC, IF, WB) LS‑C331048

Polyclonal Rabbit anti‑Human TJP1 / ZO‑1 Antibody (IHC, IF, WB) LS‑C331048

Antibody:
TJP1 / ZO-1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C331048-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TJP1 / ZO-1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
ZO-1 antibody LS-C331048 is an unconjugated rabbit polyclonal antibody to ZO-1 (TJP1) from human. It is reactive with human and mouse. Validated for Flow, IF, IHC, IP and WB.
Target
Human TJP1 / ZO-1
Synonyms
TJP1 | ZO-1 | Zona occludens protein 1 | Zona occludens 1 | Zonula occludens protein 1 | Tight junction protein 1 | ZO1 | Tight junction protein ZO-1 | Zonula occludens 1 protein
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1600-1700 of human TJP1 (NP_003248.3). PKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMC
Specificity
Human TJP1 / ZO-1
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:200 - 1:500)
  • Immunoprecipitation (1:20 - 1:50)
  • Flow Cytometry (1:20 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 186kDa/195kDa, while the observed MW by Western blot was 250kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TJP1 / ZO-1
Q07157 NM_003257 NP_003248.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024