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Catalog Number Size Price
LS-C801287-100 100 µl (1 mg/ml) $379 
LS-C801287-200 200 µl (1 mg/ml) $421 
TJP1 / ZO-1 Antibody - 1:100 staining human liver carcinoma tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
TJP1 / ZO-1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
TJP1 / ZO-1 Antibody - Western blot analysis of ZO 1 expression in human brain. The lane on the left is treated with the antigen-specific peptide.
TJP1 / ZO-1 Antibody - 1:100 staining human liver carcinoma tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
TJP1 / ZO-1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
TJP1 / ZO-1 Antibody - Western blot analysis of ZO 1 expression in human brain. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human TJP1 / ZO‑1 Antibody (IHC, IF, WB) LS‑C801287

Polyclonal Rabbit anti‑Human TJP1 / ZO‑1 Antibody (IHC, IF, WB) LS‑C801287

Antibody:
TJP1 / ZO-1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801287-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TJP1 / ZO-1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
ZO-1 antibody LS-C801287 is an unconjugated rabbit polyclonal antibody to ZO-1 (TJP1) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human TJP1 / ZO-1
Synonyms
TJP1 | ZO-1 | Zona occludens protein 1 | Zona occludens 1 | Zonula occludens protein 1 | Tight junction protein 1 | ZO1 | Tight junction protein ZO-1 | Zonula occludens 1 protein
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human ZO 1.
Specificity
ZO 1 Antibody detects endogenous levels of total ZO 1.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TJP1 / ZO-1
Q07157 NM_003257 NP_003248.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024