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Catalog Number Size Price
LS-B9009-0.05 0.05 mg (1 mg/ml) $460 
KRT5 / CK5 / Cytokeratin 5 Antibody - Anti-KRT5 / CK5 / Cytokeratin 5 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
KRT5 / CK5 / Cytokeratin 5 Antibody - Immunofluorescence of HeLa cells using CK5 mouse monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
KRT5 / CK5 / Cytokeratin 5 Antibody - Immunofluorescence of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
KRT5 / CK5 / Cytokeratin 5 Antibody - Western blot using CK5 mouse monoclonal antibody against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
KRT5 / CK5 / Cytokeratin 5 Antibody - Western blot using CK5 monoclonal antibody against human CK5 (AA: 158-272) recombinant protein. (Expected MW is 33.3 kDa)
KRT5 / CK5 / Cytokeratin 5 Antibody - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
KRT5 / CK5 / Cytokeratin 5 Antibody - Anti-KRT5 / CK5 / Cytokeratin 5 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
KRT5 / CK5 / Cytokeratin 5 Antibody - Immunofluorescence of HeLa cells using CK5 mouse monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
KRT5 / CK5 / Cytokeratin 5 Antibody - Immunofluorescence of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
KRT5 / CK5 / Cytokeratin 5 Antibody - Western blot using CK5 mouse monoclonal antibody against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
KRT5 / CK5 / Cytokeratin 5 Antibody - Western blot using CK5 monoclonal antibody against human CK5 (AA: 158-272) recombinant protein. (Expected MW is 33.3 kDa)
KRT5 / CK5 / Cytokeratin 5 Antibody - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
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PathPlus™ Monoclonal Mouse anti‑Human KRT5 / CK5 / Cytokeratin 5 Antibody (clone 2C2, aa158‑272, IHC, IF, WB) LS‑B9009

PathPlus™ Monoclonal Mouse anti‑Human KRT5 / CK5 / Cytokeratin 5 Antibody (clone 2C2, aa158‑272, IHC, IF, WB) LS‑B9009

Note: This antibody replaces LS-C169176
Antibody:
KRT5 / CK5 / Cytokeratin 5 Mouse anti-Human Monoclonal (aa158-272) (2C2) Antibody
Application:
IHC, IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B9009-0.05
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
KRT5 / CK5 / Cytokeratin 5 Mouse anti-Human Monoclonal (aa158-272) (2C2) Antibody
Application:
IHC, IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
KRT5 (Cytokeratin 5, CK5) is a protein that dimerizes with keratin 14 to form the intermediate filament that form the cytoskeleton of basal epithelial cells. It is useful for identifying myoepithelial cells and basal cells in the breast, skin, salivary glands, and prostate. It is present in the skin, the mucosa of the oral cavity, oropharynx, hypopharynx, and larynx, and in mitotically active basal cells of the skin. It is commonly positive in squamous carcinomas of the head and neck, as well as in mesotheliomas. Mutations in KRT5 cause epidermolysis bullosa simplex. Commonly used with Cytokeratin 6, the CK5/6 marker is useful for the identification of mesotheliomas as well as identifying the presence of basal epithelium in benign glands versus malignant prostate and breast carcinomas, where the basal layer is lost. References: Int J Clin Exp pathol 2008 1(1):19; Histopathology 1997 31:140; J Cell Sci 1994 107(4):765
Target
Human KRT5 / CK5 / Cytokeratin 5
Synonyms
KRT5 | 58 kd cytokeratin | 58 kDa cytokeratin | CK5 | CK-5 | Cytokeratin-5 | DDD | EBS2 | Keratin 5 | KRT5A | Type-II keratin Kb5 | Cytokeratin 5 | K5 | Keratin-5
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
2C2
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Purified recombinant fragment of human CK5 expressed in E. Coli.\
Epitope
aa158-272
Specificity
Human, Mouse, Monkey
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KRT5 / CK5 / Cytokeratin 5
P13647 NM_000424 NP_000415.2

Validation

KRT5 / CK5 / Cytokeratin 5 Antibody - Anti-KRT5 / CK5 / Cytokeratin 5 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-KRT5 / CK5 / Cytokeratin 5 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-KRT5 / CK5 / Cytokeratin 5 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

PathPlus™ KRT5 / CK5 / Cytokeratin 5 Antibody (aa158-272, clone 2C2) for IHC, IF/Immunofluorescence, WB/Western, Flow, ELISA LS-B9009 has an LSBio Rating of
Laboratory Validation Score (5)

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Request SDS/MSDS

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Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/11/2024