Target
FGF2 / Basic FGF
Synonyms
FGF2 | Basic fibroblast growth factor | FGFB | Fibroblast growth factor 2 | FGF-2 | Prostatropin | BFGF | HBGF-2
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 144 amino acid recombinant protein has a predicted molecular mass of 16,223 Da. The DTT-reduced and non-reduced protein migrate at approximately 16kDa by SDS-PAGE. The N-terminal amino acid is Alanine.
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
ED50 is equal to0.3 to 2 ng/ml, corresponding to a specific activity of 3.3 to 0.5 x 10^6 units/mg, as determined by the dose dependent stimulation of 3T3 cell proliferation.
Endotoxin
Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method).
Presentation
0.22 µm filtered protein solution, pH 7.2, 10 mM NaH2PO4, 0.5 mM EDTA, 150 mM NaCl, 1 mM DTT
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee